太湖夏季蓝藻水华期间产毒蓝藻基因型组成和种群丰度研究--《农业环境科学学报》2011年06期

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太湖夏季蓝藻水华期间产毒蓝藻基因型组成和种群丰度研究水利论文h.a5\(w-Ku(~)f

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【摘要】:采用基于mcyA基因的PCR-DGGE和定量PCR分子技术,研究了太湖夏季蓝藻水华期间不同湖区水柱和表层底泥中产毒蓝藻基因型组成和种群丰度,同时利用高效液相色谱(HPLC)法测定了水体中微囊藻毒素(Microcystin,MC)3种异构体(MC-LR、MC-YR、MC-RR)的浓度,为太湖蓝藻水华治理及其生态风险评估提供基础资料。结果表明,太湖产毒蓝藻有12种主要的基因型,不同湖区水体和底泥中基因型组成及其丰度存在差异,水体中基因型多样性高于底泥,所有样品中占优势的基因型是一致的,富营养化水平对产毒蓝藻群落结构有一定的影响。同时发现,水体中不同湖区产毒藻细胞种群丰度差异显著,富营养化水平高的湖区种群丰度也较高,底泥中产毒藻细胞种群丰度波动较小。水体中微囊藻毒素3种异构体中MC-LR浓度最高,其所占比例在湖区间有一定的差异,总微囊藻毒素浓度范围为0.54~1.08μg·L-1,部分湖区微囊藻毒素浓度超过WHO推荐的安全浓度阈值(1.0μg·L-1),必须引起足够重视。水利论文Q0gum3e$|)A

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中国科学院南京地理与湖泊研究所;中国科学院研究生院;水利论文1s]b5R2b#X

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u*N M}+a})j/^5_0蓝藻水华 定量PCR 变性梯度凝胶电泳 微囊藻毒素 太湖

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【基金】:973计划“大中型浅水湖泊蓝藻水华暴发机理研究”(2008CB418000)水利论文6]+qO6e)LY j]t

dLo3~5H,~_ l.e0中科院南京地理与湖泊研究所所长基金(07SL021001)水利论文H(yqA A3R/D%Dv

6U;c${M:o3K#i(N0【分类号】:X524水利论文:pV(B JT T

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【正文快照】:

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/o3h)Ci nB7B d0在富营养化的淡水生态系统中,蓝藻水华频繁发生已成为一个全球性的重大水环境问题,引起了广泛重视[1]。微囊藻水华是淡水水体中危害最为严重的一类,微囊藻水华发生频率高,持续时间长,且大多数产毒。微囊藻毒素(Microcystin,MCs)是由蓝藻中的微囊藻属(Microcystis)、鱼腥藻属(A

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