一株硫酸盐还原菌DSRBa的分离鉴定及特性分析--《农业环境科学学报》2011年01期

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MO'[*^a2c0一株硫酸盐还原菌DSRBa的分离鉴定及特性分析

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In6kdB6d0【摘要】:从内循环厌氧反应器颗粒污泥中分离、纯化得到一株硫酸盐还原菌命名为DSRBa,经形态和基于16SrDNA序列分析,该菌株归属于脱硫弧菌属(Desulfovibrio)。分别以甲酸钠、乙醇、乳酸钠、葡萄糖等为碳源,以硫酸盐、硫代硫酸盐、亚硫酸盐、单质硫为硫源,研究了不同温度、pH及不同硫酸根浓度对该菌株的影响。结果表明,菌株最适宜生长温度为30~35℃,最佳生长pH为7.0,无需绝对严格厌氧,当溶液中氧化还原电位(ORP)≤-40mV时,该菌株能较好生长,且生长4d后使溶液内氧化还原电位值达到-380mV,随后溶液内氧化还原电位基本保持不变。当系统内乳酸钠和酵母提取物浓度分别为3.5g·L-1和1g·L-1时,硫酸根浓度在1~4.5g·L-1范围对菌株生长无明显影响,且当SO24-浓度≤3g·L-1时,菌株生长4d对硫酸根的去除率达到90%以上。

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4S}C5p4vEU0【作者单位】:

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8^5Y c\-CNko9xj0工业聚集区污染控制与生态修复教育部重点实验室华南理工大学环境科学与工程学院;水利论文1h0?7oyp?;g*A u

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【关键词】:水利论文!n3R}~;{;Vi'W

;k&y-z$],P0硫酸盐还原菌 分离鉴定 SrDNA序列 硫酸根去除 氧化还原电位水利论文C oiH4MI

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【基金】:国家自然科学基金(40730741、41073088)水利论文 Mh8Iz gD%\

$A f)Q2ik&H0广东省自然科学基金(9351064101000001)水利论文A?5S_6@/B A

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【分类号】:X703

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【正文快照】:

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(Zu1q,wrI0化工、制药、印染、制革、造纸食品加工等工业废水中含有大量高浓度的硫酸根离子,它们对环境具有较高的潜在危害性且在环境中性质稳定,依靠自然净化作用难以去除[1]。另外在矿山废水AMD中也含有大量硫酸根离子,同样给环境带来严重的危害[2]。硫酸盐还原菌(sulphate-reducing b水利论文(]4E tZ#[0F r6| D;`,M

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