Isolation and characterization of 45 polymorphic microsatellite loci of turbot (

排行榜 收藏 打印 发给朋友 举报 来源: 《Chinese Journal of Oceanology and Limnology》 发布者:cjk3d
热度4票 浏览13次 【共0条评论】【我要评论 时间:2012年8月14日 16:03

O^q})gMtR P6U0Abstract:Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic DNA library for Scophthalmus maximus, and then isolated and characterized 45 microsatellite loci by genotyping 30individuals. The observed number of alleles ranged from 2 to 19 with an average of 6.24, while the effective number of alleles ranged from 1.30 to 11.11 with an average of 3.66. The expected heterozygosities varied from 0.235 to 0.9254 and polymorphic information content ranged from 0.2044 to 0.903 3, with an average of 0.622. Twelve loci deviated significantly from Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was observed between any pair of loci after Bonferroni correction. In cross-species amplification, five flatfish species (Paralichthys lethostigma,Verasper moseri, platichthys stellatus, Hippoglossoides dubius and Cynoglossus semilaevis) showed at least one polymorphic locus. These polymorphic microsatellite loci should prove useful for population analysis of turbot and other related species.水利论文)A@3?9t(i} x

水利论文$^ a(A4y0yY

作者:

J t'nC v ko0

Q2z Eu7t\[[6U0Author:

Y3D+J n P:_8l)w n0 水利论文#E7U9vgF)V2e$R

HOU Shiying[1] MA Aijun[2] WANG Xin'an[2] HUANG Zhihui[2] XUE Baogui[2] YANG Zhi[3] QU Jiangbo[4]水利论文-}+man3v~3b

U*d3FCL!x0作者单位:水利论文8eH7yb'A

水利论文Zs @8F M%T3y*iB

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China; Rushan Fisheries Technical Extension Station, Rushan 264500, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, ChinaRushan Fisheries Technical Extension Station, Rushan 264500, ChinaYantian Tianyuan Aquatic Limited Corporation, Yantai 264003, China水利论文#z;o Z*j"e]9b

:h*|G.w(i!}.|C'A w0期 刊:水利论文2Z.~h&QOw d L

C4B5t3T5W4BI0中国海洋湖沼学报(英文版)水利论文7{KU||

水利论文X,bDzFq+T I

Journal:

&KiS g(X \7PQ0

I7_s4MN \#J?0CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY水利论文U'T|1osE.]

水利论文1U`"f Rp"GZz

年,卷(期):水利论文~ T!TC"\E

,{d:oFs6DK02011, 29(2)水利论文^uZ{9h$x

qEN+m)F^(t2D$Y0分类号:

[9p-dC Z8At(C0 水利论文D)A w#FJ1E^5l2S

S917.4水利论文X `Dr+e5B[

S&b/{?#CAe0Keywords:水利论文6h4hp4Y^U{N8b

f[1\$g Z;TP4M7wq0turbot水利论文.b2ek$hA x

gD.SE R(Jx'{ j~3e0Scophthalmus maximus水利论文 S2L"zT:Z

&Y9|6G!bA0enriched library

%A I!b9zd0f#G'i0 水利论文Z\0`|3X!r

mierosatellite水利论文h'_o)n;D'i\

水利论文e*j9w\8M*uw

机标分类号:水利论文9K+Q/l^ `7}\,P2j

水利论文r.Z w8g'H"a s8E

TN9 TN水利论文.VH%v NM R6O

RM B!i W:O;q0机标关键词:水利论文|D"RuKU2swM

水利论文L QG~d3o"fc

microsatellite loci species linkage disequilibrium population analysis information content significant genomic DNA effective expected related study

D*aPjsh/Z0 水利论文X-nS}2d5z9a

基金项目:

dV!e'R#W4DO0

C.GViYd2p"{S ujL0the Fund for Modern Agro-lndustry Technology Research System,the National Sustainability Plan of China,the Agriculture Commonwealth Scientific Research Plan,the Yellow Sea Fisheries Research Institute Scientific and Research Fund

N+pW"n_1?v0

}T5AW1\:C/`x^U0Isolation and characterization of 45 polymorphic microsatellite loci of turbot (Scophthalmus maximus)and cross-species amplification水利论文#S i,bw'g u,O

~/J U$nS&i)z&p&G:U[P0[期刊论文] 中国海洋湖沼学报(英文版) --2011, 29(2)水利论文 x.q^` O6jh

'`J l S+x$zg-Wj`0Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic DNA library for Scophthalmus maximus, and then ...水利论文E2\c2z3S'H,OaEC

水利论文]&w/c%DgX @

返回顶部互动百科相关词

9bV(f+Z;Wj*e0
下一篇:Mitochondrial DNA control region diversity and population structure of Pacific h
上一篇:Molecular phylogeny and evolution of Scomber (Teleostei: Scombridae) based on mi
查看全部回复【已有0位网友发表了看法】

广告投放

广告投放