Isolation and characterization of 45 polymorphic microsatellite loci of turbot (

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'a-uuTD%u+Y,R#q0Abstract:Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic DNA library for Scophthalmus maximus, and then isolated and characterized 45 microsatellite loci by genotyping 30individuals. The observed number of alleles ranged from 2 to 19 with an average of 6.24, while the effective number of alleles ranged from 1.30 to 11.11 with an average of 3.66. The expected heterozygosities varied from 0.235 to 0.9254 and polymorphic information content ranged from 0.2044 to 0.903 3, with an average of 0.622. Twelve loci deviated significantly from Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was observed between any pair of loci after Bonferroni correction. In cross-species amplification, five flatfish species (Paralichthys lethostigma,Verasper moseri, platichthys stellatus, Hippoglossoides dubius and Cynoglossus semilaevis) showed at least one polymorphic locus. These polymorphic microsatellite loci should prove useful for population analysis of turbot and other related species.水利论文[@ x0k hZ S)O:g9E+t

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HOU Shiying[1] MA Aijun[2] WANG Xin'an[2] HUANG Zhihui[2] XUE Baogui[2] YANG Zhi[3] QU Jiangbo[4]水利论文)OY)D.KK(kmP1S5r

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Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China; Rushan Fisheries Technical Extension Station, Rushan 264500, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, ChinaRushan Fisheries Technical Extension Station, Rushan 264500, ChinaYantian Tianyuan Aquatic Limited Corporation, Yantai 264003, China

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中国海洋湖沼学报(英文版)水利论文sS\B)f'@+y;s6g2x

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CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY

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turbot水利论文} u2o%d7Q?iT*kE

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mierosatellite

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microsatellite loci species linkage disequilibrium population analysis information content significant genomic DNA effective expected related study水利论文I*g QI4et9d

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the Fund for Modern Agro-lndustry Technology Research System,the National Sustainability Plan of China,the Agriculture Commonwealth Scientific Research Plan,the Yellow Sea Fisheries Research Institute Scientific and Research Fund

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uI^`q$s,x_0Isolation and characterization of 45 polymorphic microsatellite loci of turbot (Scophthalmus maximus)and cross-species amplification

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0|L4MmR;j)BK0Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic DNA library for Scophthalmus maximus, and then ...

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