利用基因芯片探讨五氯酚发育毒性的作用机制 Exploration of Developmental Toxicity Mechanism of Pentach

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利用基因芯片探讨五氯酚发育毒性的作用机制

Exploration of Developmental Toxicity Mechanism of Pentachlorophenol Using cDNA Microarray

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摘要:0hpf斑马鱼胚胎经50 μg/L五氯酚(PCP)暴露染毒8 h,提取其总RNA,与Affymetrix公司的斑马鱼基因组芯片杂交.发现与对照组相比,染毒样本中1149个基因的表达显著增强(Signal log ratio>1),501个基因的表达显著减弱(Signal log ratio<-1).利用Chipinfo、Cenmapp等生物信息学软件对差异表达基因进行了基因本体论功能分类和发育调控通路分析,结果显示,显著差异表达的基因涉及抗氧化、信号传导、翻译调节、转录调节等多个分子功能.五氯酚暴露后斑马鱼胚胎发育重要的调控通路BMP信号、ERK通路、FGF信号、Nodal信号的调控基因smad2、smad5、6mp4、6mp7、flh、n-ras的表达发生了显著变化,Signal log ratio分别为4.6、2.1、1.6、1.0、1.3、1.0.这些结果为进一步阐明五氯酚的发育毒性作用机制提供了标志物.
Abstract:0hpf zebrafish embryos were exposed to 50 μg/L pentachlorophenol(PCP) for 8h in vitro .Total RNA sample was extracted then and hybridized with Affymetrix Zebrafish Genome Array representing approximately 14 900 transcripts. A total of 1 149 transcripts was significantly up-regulated while 501 transcripts were down-regulated. Bioinformatic tools were used for further analysis.The result indicated that genes with significant expression changes were related to molecular functions including antioxidant activity, signal transducer activity, translation regulator activity, transcription regulator activity ,et al. Genes regulated by BMP signals, FGF signals, and Nodal signals including smad2, smad5, bmp4, bmp7,flh , n-ras may involve in the developmental toxicity of PCP,with Signal log ratios of 4.6, 2.1, 1.6, 1.0, 1, 1.3, 1.0, respectively.This investigation may provide new biomarkers to further study of the developmental toxicity of PCP.
作 者: 吴兆毅    胡平    赵庆顺    尹大强    WU Zhao-yi    HU Ping    ZHAO Qing-shun    YIN Da-qiang  
作者单位:吴兆毅,WU Zhao-yi(南京大学环境学院,污染控制与资源化研究国家重点实验室,南京,210093)水利论文tb B5}Q
胡平,赵庆顺,HU Ping,ZHAO Qing-shun(南京大学模式动物研究所,南京,210089)水利论文RQlx.D+XO
尹大强,YIN Da-qiang(南京大学环境学院,污染控制与资源化研究国家重点实验室,南京,210093;同济大学长江水环境教育部重点实验室,上海,200092) 
期 刊:环境科学   ISTICPKU  
Journal:CHINESE JOURNAL OF ENVIRONMENTAL SCIENCE  
年,卷(期):2009, 30(11) 
分类号:X171.5 
关键词: 五氯酚    斑马鱼    基因芯片    发育毒性    生物信息学   
Keywords: pentachlorophenol    zebrafish    cDNA microarry    developmental toxicity    bioinformatics   
机标关键词:基因芯片五氯酚发育毒性毒性作用机制cDNA MicroarrayToxicity Mechanismdevelopmental toxicity差异表达基因斑马鱼胚胎antioxidant activity信号传导调控通路investigation斑马鱼基因组translationsignificant信息学软件基因本体论totalsmad5 
基金项目:国家自然科学基金 
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