聚磷菌在不同碳源下的反硝化研究 Capacity of Denitrification by Polyphosphate Accumulating Organ

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聚磷菌在不同碳源下的反硝化研究

Capacity of Denitrification by Polyphosphate Accumulating Organism at Different Electron Donors

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摘要:利用SBR系统对聚磷菌进行了培养,并通过荧光原位杂交手段检测了系统中聚磷菌 Candidatus Accumulibacter phosphatis 的富集程度.聚磷菌也是一种普通异养菌,为了研究它的反硝化能力,排除了聚磷菌的正常释磷和吸磷过程,仅考察在不同碳源下反硝化性能.结果表明,乙酸和PHB都能成为聚磷菌反硝化的电子供体.当以乙酸为外在单一碳源时,其反硝化速率和PHB生成速率与起始硝酸盐浓度无关,但是当起始状态硝酸盐浓度越高时,消耗单位乙酸生成的PHB和硝酸盐还原量越小.以PHB为内在碳源和能源时,聚磷菌的反硝化速率呈现对于基质(硝酸盐)的零级动力学反应,比反硝化速率为0.9733mg/(g·h),此外PHB平均比消耗速率为(以PHB计)2.462 6 mg/(g·h).
Abstract:SBR reactor was performed to incubate polyphosphate accumulating organism (PAO),and it was checked out of the system by fluorescence in situ hybridization. As PAO is a kind of ordinary heterotrophic bacteria, it was excluded the ability of phosphate release and uptake and it was considered only the capacity of denitrification of the target biomass. The results indicated that acetate and PHB can be the electron donors of PAO to denitrify. When fed with acetate. the denitrifying rate and PHB producing rate were independent of initial nitrate concentration. However. served as mole nitrate in the reactor, it would be less PHB produced and fewer nitrate reducing when using same amount of acetate. In view of PHB stored as all internal carbon and energy source, it presented as a reaction of zero-order to the substrate by PAO to denitrify, such as nitrate, besides, the specific denitrifying rate was 0.973 3 mg/(g·h) and the specific PHB consuming rate was 2.462 6 mg/(g·h).
作 者: 李夕耀    王淑莹    郭春艳    马勇    袁志国    彭永臻    LI Xi-yao    WANG Shu-ying    GUO Chun-yan    MA Yong    YUAN Zhi-guo    PENG Yong-zhen  
作者单位:李夕耀,王淑莹,郭春艳,马勇,彭永臻,LI Xi-yao,WANG Shu-ying,GUO Chun-yan,MA Yong,PENG Yong-zhen(北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京,100124)水利论文9b9eiGV5Ta
袁志国,YUAN Zhi-guo(Advanced,Wastewater,Management,Centre,The,University,of,Queensland,Brisbane,4072,Australia) 
期 刊:环境科学   ISTICPKU  
Journal:CHINESE JOURNAL OF ENVIRONMENTAL SCIENCE  
年,卷(期):2009, 30(10) 
分类号:X703.1 
关键词: 强化生物除磷    聚磷菌    反硝化    电子供体   
Keywords: enhanced biological phosphorus removal    polyphosphate accumulating organism (PAO)    denitfification    electron donor   
机标关键词:聚磷菌碳源反硝化速率PHB硝酸盐浓度heterotrophic bacteriaphosphate releasereactorenergy source荧光原位杂交乙酸PAOfluorescence硝酸盐还原反硝化性能动力学反应硝化能力消耗速率系统生成速率 
基金项目:国家自然科学基金,国家重大科技专项课题,北京高校人才强教计划高层次人才资助项目 
下一篇:UnFied SBR系统处理生活污水脱氮除磷的性能优化 Optimization Effect of Nitrogen and Phosphorus Remo
上一篇:纯种氨氧化细菌 Comamonas aquatic LNL_3 的固定化及短程硝化性能研究 Immobilized Ammonia-oxidizing Bac
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