PCR-DGGE技术在内源细菌选择性激活过程中的应用 Application of PCR-DGGE in Selective Activation of S

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PCR-DGGE技术在内源细菌选择性激活过程中的应用

Application of PCR-DGGE in Selective Activation of Stratal Bacteria

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摘要:分子生态学技术的发展大大促进了人们对环境中微生物群落的认识,也为研究油藏微生物群落提供了一个新的工具.在模拟油藏条件下,应用PCR-DGGE技术研究激活剂的注入对油藏微生物群落结构的影响,考察了激活过程中菌群结构的变化情况.分析了常压(1 MPa)和高压(10 MPa)下内源激活DGGE条带变化,进行了激活过程中的PCR序列分析,考察了激活不同阶段中的优势菌,将优势条带序列构建系统发育树,并对激活过程中主要内源菌所在类群分析.结果表明,高压条件下,优势菌群结构与常压下差异较大,常压激活和高压激活细菌类群数量变化差异显著;激活后,优势菌的种类有所增加,群落结构也发生了变化.激活前,优势菌是芽孢杆菌,而激活剂的加入使变形菌的在种类上得到增加.
作 者: 包木太    王兵    陈庆国    高光军    李希明    BAO Mu-tai    WANG Bing    CHEN Qing-guo    GAO Guang-jun    LI Xi-ming  
作者单位:包木太,王兵,陈庆国,BAO Mu-tai,WANG Bing,CHEN Qing-guo(中国海洋大学海洋化学理论与工程技术教育部重点实验室,青岛,266100)
:y!Z@ V!Z+v1hR6ax8P0高光军,李希明,GAO Guang-jun,LI Xi-ming(中国石化胜利油田分公司采油工艺研究院,东营,257000) 
期 刊:环境科学   ISTICPKU  
Journal:CHINESE JOURNAL OF ENVIRONMENTAL SCIENCE  
年,卷(期):2009, 30(6) 
分类号:X172 
关键词: 内源细菌    选择性激活    DGGE    PCR测序   
机标关键词:PCR-DGGE技术内源细菌类群选择性激活过程油藏微生物群落优势菌微生物群落结构菌群结构激活剂高压分析常压系统发育树种类油藏条件芽孢杆菌PCR序列条带数量变化 
基金项目:国家自然科学基金 
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