乐果降解菌LGX1的筛选及其降解特性研究 Study on screening and characteristics of a dimethoate-deg

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乐果降解菌LGX1的筛选及其降解特性研究

Study on screening and characteristics of a dimethoate-degrading bacterium strain LGX1

通过富集培养,从连续施用农药乐果的土壤中分离得到一株具有较强降解有机磷农药乐果能力的细菌菌株LGX1,通过菌落形态观察及细菌的16S rDNA测序,对其进行了鉴定,同时初步研究了其降解性能.结果表明:该菌株为蜡状芽孢杆菌(Bacillus cereus),在接种量为20%,乐果初始质量浓度为100 mg·L-1,外加碳源葡萄糖质量分数为2%,温度为35℃,初始pH值为4时该菌株对乐果的降解能力最强;并且能在以辛硫磷、毒死蜱和三唑磷为唯一碳源的基础盐培养基中生长,且均比在以乐果为唯一碳源的基础盐培养基中生长的OD600值要高,初步推断菌株LGX1对有机磷农药的降解有一定的广谱性.水利论文CM/L6ov

作 者: 邓晓 李勤奋 侯宪文 李光义 DENG Xiao LI Qinfen HOU Xianwen LI Guangyi  
作者单位:中国热带农业科学院环境与植物保护研究所,海南,儋州,571737 
刊 名:生态环境学报  ISTIC PKU
英文刊名:ECOLOGY AND ENVIRONMENTAL SCIENCES 
年,卷(期):2010 19(5) 
分类号:X172 
关键词:乐果   降解菌   筛选   降解性能  
机标关键词:乐果降解菌筛选特性研究bacterium strain有机磷农药细菌菌株外加碳源培养基蜡状芽孢杆菌质量浓度质量分数形态观察生长降解能力连续施用降解性能基础富集培养初步研究 
基金项目:中国热带农业科学院基金项目 
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